Review



human light tnsf14 quantikine elisa kit  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    R&D Systems human light tnsf14 quantikine elisa kit
    Human Light Tnsf14 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+light+tnfsf14/Human+LIGHT%2FTNFSF14+Quantikine+ELISA+Kit/us12594294-359-38-44
    Average 94 stars, based on 8 article reviews
    human light tnsf14 quantikine elisa kit - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Serum soluble LIGHT in the early third trimester as a novel biomarker for predicting late-onset preeclampsia.
    Article Snippet: Our aim was to evaluate whether serum levels of soluble LIGHT (sLIGHT) at 27–31weeks can predict the later occurrence of gestational hypertension (GH), late-onset preeclampsia (PE), and early-onset PE.. Mean blood pressure (MBP), soluble fma-like tyrosine kinase 1/placental growth factor (sFlt-1/PlGF) ratio at 27–31 weeks, and sLIGHT at 27–31weeks were independent risk factors for late-onset PE.. The combination of the three risk factors improved sensitivity with a false-positive rate of 10% (MBP: 60%, log10(sFlt-1/PlGF): 45%, sLIGHT: 35%, combination: 75%).

    Recombinant:

    Article Title: Effect of Tumor Necrosis Factor Family Member LIGHT (TNFSF14) on the Activation of Basophils and Eosinophils Interacting with Bronchial Epithelial Cells
    Article Snippet: .. The recombinant human LIGHT/TNFSF14 was purchased from R&D Systems (Minneapolis, MN, USA). .. I κ B α phosphorylation inhibitor BAY11-7082, p38 MAPK inhibitor SB203580, c-Jun N-terminal protein kinase (JNK) inhibitor SP600125, extracellular signal-regulated kinase (ERK) inhibitor U0126, and PI3K inhibitor LY294002 were purchased from Calbiochem Corporation (San Diego, CA, USA).



    Similar Products

    il 6  (Multi Sciences (Lianke) Biotech Co Ltd)
    94
    Multi Sciences (Lianke) Biotech Co Ltd il 6
    H1N1 infection affects cell viability, inflammatory cytokine secretion and interactions between HBEpiCs and THP-1 cells. (A) CCK-8 assay revealed that HBEpiC viability decreased in a concentration-dependent manner following H1N1 infection. (B) ELISA revealed that the levels of IL-1β, <t>IL-6,</t> TNF-α, and IL-8 in HBEpiCs decreased with increasing H1N1 infection. (C) CCK-8 assay indicated that supernatants from H1N1-infected HBEpiC cultures reduced the viability of THP-1 cells in a dose-dependent manner. (D) ELISA results suggested that the levels of inflammatory cytokines (IL-1β, IL-6, TNF-α and IL-8) in THP-1 cells were decreased following exposure to supernatants from H1N1-infected HBEpiC cultures. (E) Cell adhesion assay revealed that the number of THP-1 cells adhering to HBEpiCs increased with increasing H1N1 concentration (scale bar, 10 μ m). Arrow indicates THP-1 cells that remain attached to the surface of HBEpiCs, highlighting the adhesion interaction between the two cell types. (F) Transwell assay suggested that H1N1 infection enhanced the migration capacity of THP-1 cells, with increased migration observed at higher virus concentrations (scale bar, 50 μ m). The data are presented as the mean ± standard deviation; ** P<0.01, *** P<0.001 vs. control. H1N1, influenza A; HBEpiCs, human bronchial epithelial cells; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; TNF-α, tumor necrosis factor-α; Con, control; MOI, multiplicity of infection.
    Il 6, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+light+tnfsf14/Human+CD258%2FLIGHT%2FTNFSF14+ELISA+Kit/pmc13105392-65-51-56
    Average 94 stars, based on 1 article reviews
    il 6 - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    tfs target  (TargetMol)
    94
    TargetMol tfs target
    H1N1 infection affects cell viability, inflammatory cytokine secretion and interactions between HBEpiCs and THP-1 cells. (A) CCK-8 assay revealed that HBEpiC viability decreased in a concentration-dependent manner following H1N1 infection. (B) ELISA revealed that the levels of IL-1β, <t>IL-6,</t> TNF-α, and IL-8 in HBEpiCs decreased with increasing H1N1 infection. (C) CCK-8 assay indicated that supernatants from H1N1-infected HBEpiC cultures reduced the viability of THP-1 cells in a dose-dependent manner. (D) ELISA results suggested that the levels of inflammatory cytokines (IL-1β, IL-6, TNF-α and IL-8) in THP-1 cells were decreased following exposure to supernatants from H1N1-infected HBEpiC cultures. (E) Cell adhesion assay revealed that the number of THP-1 cells adhering to HBEpiCs increased with increasing H1N1 concentration (scale bar, 10 μ m). Arrow indicates THP-1 cells that remain attached to the surface of HBEpiCs, highlighting the adhesion interaction between the two cell types. (F) Transwell assay suggested that H1N1 infection enhanced the migration capacity of THP-1 cells, with increased migration observed at higher virus concentrations (scale bar, 50 μ m). The data are presented as the mean ± standard deviation; ** P<0.01, *** P<0.001 vs. control. H1N1, influenza A; HBEpiCs, human bronchial epithelial cells; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; TNF-α, tumor necrosis factor-α; Con, control; MOI, multiplicity of infection.
    Tfs Target, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+light+tnfsf14/LIGHT-TNFSF14+Trimer+Protein%2C+Human%2C+Recombinant/pm41789592-234-87-370
    Average 94 stars, based on 1 article reviews
    tfs target - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    tnfsf14 elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)
    94
    Multi Sciences (Lianke) Biotech Co Ltd tnfsf14 elisa kit
    H1N1 infection affects cell viability, inflammatory cytokine secretion and interactions between HBEpiCs and THP-1 cells. (A) CCK-8 assay revealed that HBEpiC viability decreased in a concentration-dependent manner following H1N1 infection. (B) ELISA revealed that the levels of IL-1β, <t>IL-6,</t> TNF-α, and IL-8 in HBEpiCs decreased with increasing H1N1 infection. (C) CCK-8 assay indicated that supernatants from H1N1-infected HBEpiC cultures reduced the viability of THP-1 cells in a dose-dependent manner. (D) ELISA results suggested that the levels of inflammatory cytokines (IL-1β, IL-6, TNF-α and IL-8) in THP-1 cells were decreased following exposure to supernatants from H1N1-infected HBEpiC cultures. (E) Cell adhesion assay revealed that the number of THP-1 cells adhering to HBEpiCs increased with increasing H1N1 concentration (scale bar, 10 μ m). Arrow indicates THP-1 cells that remain attached to the surface of HBEpiCs, highlighting the adhesion interaction between the two cell types. (F) Transwell assay suggested that H1N1 infection enhanced the migration capacity of THP-1 cells, with increased migration observed at higher virus concentrations (scale bar, 50 μ m). The data are presented as the mean ± standard deviation; ** P<0.01, *** P<0.001 vs. control. H1N1, influenza A; HBEpiCs, human bronchial epithelial cells; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; TNF-α, tumor necrosis factor-α; Con, control; MOI, multiplicity of infection.
    Tnfsf14 Elisa Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+light+tnfsf14/Human+CD258%2FLIGHT%2FTNFSF14+ELISA+Kit/pm42033552-59-6-9
    Average 94 stars, based on 1 article reviews
    tnfsf14 elisa kit - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    human light tnsf14 quantikine elisa kit  (R&D Systems)
    94
    R&D Systems human light tnsf14 quantikine elisa kit
    H1N1 infection affects cell viability, inflammatory cytokine secretion and interactions between HBEpiCs and THP-1 cells. (A) CCK-8 assay revealed that HBEpiC viability decreased in a concentration-dependent manner following H1N1 infection. (B) ELISA revealed that the levels of IL-1β, <t>IL-6,</t> TNF-α, and IL-8 in HBEpiCs decreased with increasing H1N1 infection. (C) CCK-8 assay indicated that supernatants from H1N1-infected HBEpiC cultures reduced the viability of THP-1 cells in a dose-dependent manner. (D) ELISA results suggested that the levels of inflammatory cytokines (IL-1β, IL-6, TNF-α and IL-8) in THP-1 cells were decreased following exposure to supernatants from H1N1-infected HBEpiC cultures. (E) Cell adhesion assay revealed that the number of THP-1 cells adhering to HBEpiCs increased with increasing H1N1 concentration (scale bar, 10 μ m). Arrow indicates THP-1 cells that remain attached to the surface of HBEpiCs, highlighting the adhesion interaction between the two cell types. (F) Transwell assay suggested that H1N1 infection enhanced the migration capacity of THP-1 cells, with increased migration observed at higher virus concentrations (scale bar, 50 μ m). The data are presented as the mean ± standard deviation; ** P<0.01, *** P<0.001 vs. control. H1N1, influenza A; HBEpiCs, human bronchial epithelial cells; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; TNF-α, tumor necrosis factor-α; Con, control; MOI, multiplicity of infection.
    Human Light Tnsf14 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+light+tnfsf14/Human+LIGHT%2FTNFSF14+Quantikine+ELISA+Kit/us12594294-359-38-44
    Average 94 stars, based on 1 article reviews
    human light tnsf14 quantikine elisa kit - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    recombinant human light tnfsf14 protein  (R&D Systems)
    94
    R&D Systems recombinant human light tnfsf14 protein
    H1N1 infection affects cell viability, inflammatory cytokine secretion and interactions between HBEpiCs and THP-1 cells. (A) CCK-8 assay revealed that HBEpiC viability decreased in a concentration-dependent manner following H1N1 infection. (B) ELISA revealed that the levels of IL-1β, <t>IL-6,</t> TNF-α, and IL-8 in HBEpiCs decreased with increasing H1N1 infection. (C) CCK-8 assay indicated that supernatants from H1N1-infected HBEpiC cultures reduced the viability of THP-1 cells in a dose-dependent manner. (D) ELISA results suggested that the levels of inflammatory cytokines (IL-1β, IL-6, TNF-α and IL-8) in THP-1 cells were decreased following exposure to supernatants from H1N1-infected HBEpiC cultures. (E) Cell adhesion assay revealed that the number of THP-1 cells adhering to HBEpiCs increased with increasing H1N1 concentration (scale bar, 10 μ m). Arrow indicates THP-1 cells that remain attached to the surface of HBEpiCs, highlighting the adhesion interaction between the two cell types. (F) Transwell assay suggested that H1N1 infection enhanced the migration capacity of THP-1 cells, with increased migration observed at higher virus concentrations (scale bar, 50 μ m). The data are presented as the mean ± standard deviation; ** P<0.01, *** P<0.001 vs. control. H1N1, influenza A; HBEpiCs, human bronchial epithelial cells; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; TNF-α, tumor necrosis factor-α; Con, control; MOI, multiplicity of infection.
    Recombinant Human Light Tnfsf14 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+light+tnfsf14/Recombinant+Human+LIGHT%2FTNFSF14+Protein/pm41833106-49-14-21
    Average 94 stars, based on 1 article reviews
    recombinant human light tnfsf14 protein - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    recombinant human light  (R&D Systems)
    94
    R&D Systems recombinant human light
    A. The abundance of total IHL isolated from WT and Il-17a -/- mice is quantified (N = 4-6 per group). B. Total number of IHL isolated from WT and Il-17a -/- mice reared on control or DDC diet is normalized to liver weight (N = 4-6 per group). C. The proportion of CD45 + cells in the IHL as determined by flow cytometry (N = 4-6 per group). D. (Left panel) CD4 + T cells abundance in gated CD45 + immune cells, and (Right panel ) the proportion of <t>LIGHT</t> + cells in the CD4 + T cell fraction (N = 3 per group). E. (Left panel) NK T cell abundance in gated CD45 + immune cells, and (Right panel ) proportion of LIGHT + cells in the NK T cell fraction (N = 4-6 per group). F. LIGHT positivity in CD4 + T cell subsets, Th1, Th2, Th17, and T-reg, was determined by flow cytometry (N = 3 per group). G. WT primary mouse splenocytes when stimulated with PMA/ionomycin or vehicle display (left panel) a similar proportion of CD3 + CD4 + T cells, (middle panel) an increased abundance of IFNγ + CD 4 + T cells and (right panel) an increased proportion of LIGHT + CD4 + T cells (N = 3 per group). H. The expression of pro-fibrogenic genes ACTA2, COL1A1, PDGFRA and CTGF is elevated in primary human hepatic stellate cells when stimulated with <t>recombinant</t> LIGHT (N = 3 per group). * - p < 0.05, ** - p < 0.01, *** - p < 0.005, **** - p < 0.001.
    Recombinant Human Light, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+light+tnfsf14/Recombinant+Human+LIGHT%2FTNFSF14+Protein/pmc12880643-91-13-17
    Average 94 stars, based on 1 article reviews
    recombinant human light - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    light 664 li  (R&D Systems)
    94
    R&D Systems light 664 li
    A. The abundance of total IHL isolated from WT and Il-17a -/- mice is quantified (N = 4-6 per group). B. Total number of IHL isolated from WT and Il-17a -/- mice reared on control or DDC diet is normalized to liver weight (N = 4-6 per group). C. The proportion of CD45 + cells in the IHL as determined by flow cytometry (N = 4-6 per group). D. (Left panel) CD4 + T cells abundance in gated CD45 + immune cells, and (Right panel ) the proportion of <t>LIGHT</t> + cells in the CD4 + T cell fraction (N = 3 per group). E. (Left panel) NK T cell abundance in gated CD45 + immune cells, and (Right panel ) proportion of LIGHT + cells in the NK T cell fraction (N = 4-6 per group). F. LIGHT positivity in CD4 + T cell subsets, Th1, Th2, Th17, and T-reg, was determined by flow cytometry (N = 3 per group). G. WT primary mouse splenocytes when stimulated with PMA/ionomycin or vehicle display (left panel) a similar proportion of CD3 + CD4 + T cells, (middle panel) an increased abundance of IFNγ + CD 4 + T cells and (right panel) an increased proportion of LIGHT + CD4 + T cells (N = 3 per group). H. The expression of pro-fibrogenic genes ACTA2, COL1A1, PDGFRA and CTGF is elevated in primary human hepatic stellate cells when stimulated with <t>recombinant</t> LIGHT (N = 3 per group). * - p < 0.05, ** - p < 0.01, *** - p < 0.005, **** - p < 0.001.
    Light 664 Li, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+light+tnfsf14/Recombinant+Human+LIGHT%2FTNFSF14+Protein/pmc12580646-24-118-122
    Average 94 stars, based on 1 article reviews
    light 664 li - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    rlight  (R&D Systems)
    94
    R&D Systems rlight
    A. The abundance of total IHL isolated from WT and Il-17a -/- mice is quantified (N = 4-6 per group). B. Total number of IHL isolated from WT and Il-17a -/- mice reared on control or DDC diet is normalized to liver weight (N = 4-6 per group). C. The proportion of CD45 + cells in the IHL as determined by flow cytometry (N = 4-6 per group). D. (Left panel) CD4 + T cells abundance in gated CD45 + immune cells, and (Right panel ) the proportion of <t>LIGHT</t> + cells in the CD4 + T cell fraction (N = 3 per group). E. (Left panel) NK T cell abundance in gated CD45 + immune cells, and (Right panel ) proportion of LIGHT + cells in the NK T cell fraction (N = 4-6 per group). F. LIGHT positivity in CD4 + T cell subsets, Th1, Th2, Th17, and T-reg, was determined by flow cytometry (N = 3 per group). G. WT primary mouse splenocytes when stimulated with PMA/ionomycin or vehicle display (left panel) a similar proportion of CD3 + CD4 + T cells, (middle panel) an increased abundance of IFNγ + CD 4 + T cells and (right panel) an increased proportion of LIGHT + CD4 + T cells (N = 3 per group). H. The expression of pro-fibrogenic genes ACTA2, COL1A1, PDGFRA and CTGF is elevated in primary human hepatic stellate cells when stimulated with <t>recombinant</t> LIGHT (N = 3 per group). * - p < 0.05, ** - p < 0.01, *** - p < 0.005, **** - p < 0.001.
    Rlight, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+light+tnfsf14/Recombinant+Human+LIGHT%2FTNFSF14+Protein/pmc12448905-22-7-5
    Average 94 stars, based on 1 article reviews
    rlight - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    H1N1 infection affects cell viability, inflammatory cytokine secretion and interactions between HBEpiCs and THP-1 cells. (A) CCK-8 assay revealed that HBEpiC viability decreased in a concentration-dependent manner following H1N1 infection. (B) ELISA revealed that the levels of IL-1β, IL-6, TNF-α, and IL-8 in HBEpiCs decreased with increasing H1N1 infection. (C) CCK-8 assay indicated that supernatants from H1N1-infected HBEpiC cultures reduced the viability of THP-1 cells in a dose-dependent manner. (D) ELISA results suggested that the levels of inflammatory cytokines (IL-1β, IL-6, TNF-α and IL-8) in THP-1 cells were decreased following exposure to supernatants from H1N1-infected HBEpiC cultures. (E) Cell adhesion assay revealed that the number of THP-1 cells adhering to HBEpiCs increased with increasing H1N1 concentration (scale bar, 10 μ m). Arrow indicates THP-1 cells that remain attached to the surface of HBEpiCs, highlighting the adhesion interaction between the two cell types. (F) Transwell assay suggested that H1N1 infection enhanced the migration capacity of THP-1 cells, with increased migration observed at higher virus concentrations (scale bar, 50 μ m). The data are presented as the mean ± standard deviation; ** P<0.01, *** P<0.001 vs. control. H1N1, influenza A; HBEpiCs, human bronchial epithelial cells; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; TNF-α, tumor necrosis factor-α; Con, control; MOI, multiplicity of infection.

    Journal: International Journal of Molecular Medicine

    Article Title: Triptolide exerts antiviral effects and alleviates influenza A-induced pneumonia by inhibiting the overactivation of absent in melanoma 2 signaling in immune cells

    doi: 10.3892/ijmm.2026.5829

    Figure Lengend Snippet: H1N1 infection affects cell viability, inflammatory cytokine secretion and interactions between HBEpiCs and THP-1 cells. (A) CCK-8 assay revealed that HBEpiC viability decreased in a concentration-dependent manner following H1N1 infection. (B) ELISA revealed that the levels of IL-1β, IL-6, TNF-α, and IL-8 in HBEpiCs decreased with increasing H1N1 infection. (C) CCK-8 assay indicated that supernatants from H1N1-infected HBEpiC cultures reduced the viability of THP-1 cells in a dose-dependent manner. (D) ELISA results suggested that the levels of inflammatory cytokines (IL-1β, IL-6, TNF-α and IL-8) in THP-1 cells were decreased following exposure to supernatants from H1N1-infected HBEpiC cultures. (E) Cell adhesion assay revealed that the number of THP-1 cells adhering to HBEpiCs increased with increasing H1N1 concentration (scale bar, 10 μ m). Arrow indicates THP-1 cells that remain attached to the surface of HBEpiCs, highlighting the adhesion interaction between the two cell types. (F) Transwell assay suggested that H1N1 infection enhanced the migration capacity of THP-1 cells, with increased migration observed at higher virus concentrations (scale bar, 50 μ m). The data are presented as the mean ± standard deviation; ** P<0.01, *** P<0.001 vs. control. H1N1, influenza A; HBEpiCs, human bronchial epithelial cells; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; TNF-α, tumor necrosis factor-α; Con, control; MOI, multiplicity of infection.

    Article Snippet: Cell supernatants were collected and analyzed using Human TNF-α High Sensitivity ELISA Kit [cat. no. EK182HS; Hangzhou Multi Sciences (Lianke) Biotech Co., Ltd.], Human IL-8 ELISA Kit [cat. no. EK108; Hangzhou Multi Sciences (Lianke) Biotech Co., Ltd.], a human IL-1β ELISA kit [EH0185; Hangzhou Multi Sciences (Lianke) Biotech Co., Ltd.] and IL-6 [cat. no. EK1217; Hangzhou Multi Sciences (Lianke) Biotech Co., Ltd.] according to the manufacturer's instructions.

    Techniques: Infection, CCK-8 Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Cell Adhesion Assay, Transwell Assay, Migration, Virus, Standard Deviation, Control

    TP modulates the inflammatory response and immune cell activity in H1N1-infected HBEpiCs and THP-1 cells. (A) No significant changes were observed in HBEpiCs treated with various concentrations of TP (5, 10 and 20 nM) following H1N1 infection compared with the control. (B) After TP treatment, the levels of the inflammatory cytokines IL-1β, IL-6, TNF-α and IL-8 in HBEpiCs were markedly lower than those in the untreated group. (C) The viability of THP-1 cells pretreated with H1N1-infected HBEpiC culture supernatant decreased after TP treatment. (D) The levels of IL-1β, IL-6, TNF-α, and IL-8 in THP-1 cells were markedly lower after TP treatment. (E) The adhesion of THP-1 cells to HBEpiCs induced by H1N1 infection decreased in a dose-dependent manner with increasing TP concentration (scale bar, 10 μ m). Arrow indicates THP-1 cells that remain attached to the surface of HBEpiCs, highlighting the adhesion interaction between the two cell types. (F) The migration capacity of THP-1 cells was markedly reduced when the supernatant from H1N1-infected HBEpiC cultures was treated with TP (scale bar, 50 μ m). The data are presented as the mean ± standard deviation; * P<0.05, ** P<0.01, *** P<0.001 vs. control. TP, triptolide; H1N1, influenza A; HBEpiCs, human bronchial epithelial cells; IL, interleukin; TNF-α, tumor necrosis factor-α; Con, control.

    Journal: International Journal of Molecular Medicine

    Article Title: Triptolide exerts antiviral effects and alleviates influenza A-induced pneumonia by inhibiting the overactivation of absent in melanoma 2 signaling in immune cells

    doi: 10.3892/ijmm.2026.5829

    Figure Lengend Snippet: TP modulates the inflammatory response and immune cell activity in H1N1-infected HBEpiCs and THP-1 cells. (A) No significant changes were observed in HBEpiCs treated with various concentrations of TP (5, 10 and 20 nM) following H1N1 infection compared with the control. (B) After TP treatment, the levels of the inflammatory cytokines IL-1β, IL-6, TNF-α and IL-8 in HBEpiCs were markedly lower than those in the untreated group. (C) The viability of THP-1 cells pretreated with H1N1-infected HBEpiC culture supernatant decreased after TP treatment. (D) The levels of IL-1β, IL-6, TNF-α, and IL-8 in THP-1 cells were markedly lower after TP treatment. (E) The adhesion of THP-1 cells to HBEpiCs induced by H1N1 infection decreased in a dose-dependent manner with increasing TP concentration (scale bar, 10 μ m). Arrow indicates THP-1 cells that remain attached to the surface of HBEpiCs, highlighting the adhesion interaction between the two cell types. (F) The migration capacity of THP-1 cells was markedly reduced when the supernatant from H1N1-infected HBEpiC cultures was treated with TP (scale bar, 50 μ m). The data are presented as the mean ± standard deviation; * P<0.05, ** P<0.01, *** P<0.001 vs. control. TP, triptolide; H1N1, influenza A; HBEpiCs, human bronchial epithelial cells; IL, interleukin; TNF-α, tumor necrosis factor-α; Con, control.

    Article Snippet: Cell supernatants were collected and analyzed using Human TNF-α High Sensitivity ELISA Kit [cat. no. EK182HS; Hangzhou Multi Sciences (Lianke) Biotech Co., Ltd.], Human IL-8 ELISA Kit [cat. no. EK108; Hangzhou Multi Sciences (Lianke) Biotech Co., Ltd.], a human IL-1β ELISA kit [EH0185; Hangzhou Multi Sciences (Lianke) Biotech Co., Ltd.] and IL-6 [cat. no. EK1217; Hangzhou Multi Sciences (Lianke) Biotech Co., Ltd.] according to the manufacturer's instructions.

    Techniques: Activity Assay, Infection, Control, Concentration Assay, Migration, Standard Deviation

    A. The abundance of total IHL isolated from WT and Il-17a -/- mice is quantified (N = 4-6 per group). B. Total number of IHL isolated from WT and Il-17a -/- mice reared on control or DDC diet is normalized to liver weight (N = 4-6 per group). C. The proportion of CD45 + cells in the IHL as determined by flow cytometry (N = 4-6 per group). D. (Left panel) CD4 + T cells abundance in gated CD45 + immune cells, and (Right panel ) the proportion of LIGHT + cells in the CD4 + T cell fraction (N = 3 per group). E. (Left panel) NK T cell abundance in gated CD45 + immune cells, and (Right panel ) proportion of LIGHT + cells in the NK T cell fraction (N = 4-6 per group). F. LIGHT positivity in CD4 + T cell subsets, Th1, Th2, Th17, and T-reg, was determined by flow cytometry (N = 3 per group). G. WT primary mouse splenocytes when stimulated with PMA/ionomycin or vehicle display (left panel) a similar proportion of CD3 + CD4 + T cells, (middle panel) an increased abundance of IFNγ + CD 4 + T cells and (right panel) an increased proportion of LIGHT + CD4 + T cells (N = 3 per group). H. The expression of pro-fibrogenic genes ACTA2, COL1A1, PDGFRA and CTGF is elevated in primary human hepatic stellate cells when stimulated with recombinant LIGHT (N = 3 per group). * - p < 0.05, ** - p < 0.01, *** - p < 0.005, **** - p < 0.001.

    Journal: PLOS One

    Article Title: Genetic ablation of interleukin-17A augments fibrosis in a mouse model of cholestatic liver injury

    doi: 10.1371/journal.pone.0342251

    Figure Lengend Snippet: A. The abundance of total IHL isolated from WT and Il-17a -/- mice is quantified (N = 4-6 per group). B. Total number of IHL isolated from WT and Il-17a -/- mice reared on control or DDC diet is normalized to liver weight (N = 4-6 per group). C. The proportion of CD45 + cells in the IHL as determined by flow cytometry (N = 4-6 per group). D. (Left panel) CD4 + T cells abundance in gated CD45 + immune cells, and (Right panel ) the proportion of LIGHT + cells in the CD4 + T cell fraction (N = 3 per group). E. (Left panel) NK T cell abundance in gated CD45 + immune cells, and (Right panel ) proportion of LIGHT + cells in the NK T cell fraction (N = 4-6 per group). F. LIGHT positivity in CD4 + T cell subsets, Th1, Th2, Th17, and T-reg, was determined by flow cytometry (N = 3 per group). G. WT primary mouse splenocytes when stimulated with PMA/ionomycin or vehicle display (left panel) a similar proportion of CD3 + CD4 + T cells, (middle panel) an increased abundance of IFNγ + CD 4 + T cells and (right panel) an increased proportion of LIGHT + CD4 + T cells (N = 3 per group). H. The expression of pro-fibrogenic genes ACTA2, COL1A1, PDGFRA and CTGF is elevated in primary human hepatic stellate cells when stimulated with recombinant LIGHT (N = 3 per group). * - p < 0.05, ** - p < 0.01, *** - p < 0.005, **** - p < 0.001.

    Article Snippet: Cells were rested for 4 hours and stimulated with medium containing vehicle or recombinant human LIGHT (664-LI, R&D systems, Minneapolis, MN) for 72 hours refreshing the medium at 48 hours.

    Techniques: Isolation, Control, Flow Cytometry, Expressing, Recombinant